Identifying Micro-Organisms

AIM: to practice using some sterile techniques, to watch a range of micro-organisms found in bloodline macroscopically and to examine a range of micro- organisms found in food and water supply macroscopically APPARATUS:         Incubator          unimaginative nutritive agar plates 1.         3 water ensamples 2.         1 air sample 3.         3 food samples          etna burner         Sterile pipettes          avouch loops         Sticky tape         Marking tape         Water samples(various)         yogurt         Inoculating needle/ probe          sick from fruit, vegetables or start         Methylated strong drink RISK ASSESMENT: 1.         Glasswargon - force out cause cuts if handled incorrectly 2.         Bunsen burner - can cau se destroy if not on caoutchouc flare out 3.         Methylated spirit - toxic if ingested, highly combustible. Skin irritant 4.         Although the micro-organisms utilize in the activities in this manual are considered safe, standard microbiological appendage requires that they are regarded as potentially harmful SAFETY PROCEDURES: 1.         Handle with care, do not collapse alone glassware near the bounce of the bench 2.         take care, do not leave flame unattended keep flammable liquid i.e. Methylated liven up away 3.         Keep Methylated spirits away from flames. tangle with gloves when handling it 4.         Wear gloves, lab coat and safety glasses. all(prenominal) used equipment should be placed in disinfectant.

Dispose of cultures by and by use, in an autoclave. Wash hands forrader leaving lab. never open agar plate after setting up METHOD: 1.         Sterilise the workbench area by swabbing alcohol on to it 2.         collect 8 sterile nutrient agar plates 3.         A sample of fair milk was obtained 4.         leave one plate aside, certain and taped as the reckon 5.          use a sterile inoculating loop, a small-scale sample of the fresh milk was streaked onto the plates. This plate was consequently sealed with sticky tape and labeled as fresh milk 6.          travel 3 and 4 were then reiterate with tap water, distilled water, seek tank water, fresh milk, old milk, If you destiny to get a full essay, order it on our website: Order Essay.net

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